GordoTEK
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Making and Using a Spore Syringe
🕑 Added 2022-01-31 04:55:05 +0000 UTCIn this video I show the essential steps not shown in my pan cyan video, that is, how to generate the grain spawn if all you have is a spore print. This video was requested by a patron, hope you enjoy it. It is also available on Rumble and on Odysee. Using a spore syringe is an easier alternative to using agar.
Note: some people say you should wait 24 hours before using a freshly made spore syringe to give the spores time to fully hydrate. I consider this idea debunked mythology. Anyone who has ever done any spore work with a microscope knows the dry spores fully hydrate in minutes if not seconds, you can actually watch it happen under the microscope. They most certainly do NOT need 24 hours. I've made plenty of spore syringes where I did not wait at all between creating the syringe and injecting it to grain jars, and they worked perfectly. In fact I actually RECOMMEND using them as fast as possible, why give any potential contam or bacteria a chance to grow in the water? Just get those spores on grain as soon as possible and get it into an incubator.
[Note: If you use any of my Amazon links it supports the channel! (“As an Amazon Associate I earn from qualifying purchases.”)]
https://amzn.to/3ABkdmP (Sterile Syringes, luer lock)
https://amzn.to/3g7wVQP (optional syringe tip caps, luer lock)
https://amzn.to/3IOdFUu (Lids)
https://amzn.to/35zcPgf (mycobags)
https://amzn.to/3IPr5Q7 (presto sterilizer)
https://midwestgrowkits.referralrock.com/l/1GORDOTEK90/ (Look under “Substrate Jars”)
Whole Oats: https://www.tractorsupply.com/tsc/product/producers-pride-oats-50-lb
Or on amazon for much higher price: https://amzn.to/3rT0NWk
https://amzn.to/34hnITg (Polyfill, cheaper at walmart or cut open a stuffed animal)
https://amzn.to/3KYHcN8 (16 oz mason jars)
https://amzn.to/3gaPYtt (hygrometer/thermometer)
I show how to make and use a still air box in this vid: https://www.patreon.com/posts/29675383
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Links to all of my TEKs, Videos, Trip music, and frequently asked questions with answers can be found here: https://www.patreon.com/posts/22774475 (everything is made available for FREE, I do not use pay walls, support the work only if you feel like you've benefited from it and you are able to do so).
Social media:
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Comments
Kopo Red
No for instance if you have a agar plate with contamination. And want to clean it up. You must use a SAB. A flow hood can de disastrous in this situation.
Wazatron
Does using a laminar flow hood completely eliminate the need to work in a still air box? I believe so, but I'm hoping to check my work to make sure I'm not making a silly mistake planning things out.
james Sutton
I
Riverchick
Thank you for sharing your tuition and experience. I would love a spore print.
